Coding

Part:BBa_M11026:Design

Designed by: Trent Mortensen   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-16)

Beta-glucosidase (bgl 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 49
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2238
    Illegal NgoMIV site found at 2254
    Illegal AgeI site found at 1234
    Illegal AgeI site found at 2314
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence for beta-glucosidase was back-translated using DNA 2.0 to a DNA sequence optimized for use in E. coli. The DNA sequence then had four Pst1 (ctgcag) sites at bp 35, 1984, 2098, and 2413. These sites were mutated accordingly: the 35 site to ctgcgg, the 1984 site to ctgcaa, the 2098 site to ctgcaa, and the 2413 site to ctgcaa. Two stop codons were then added to the sequence (taataa).

Source

This enzyme is coded for in a number of organisms, but was taken from Aspergillus niger in this case.

References